| Share facebook twitter | RSS

Ambassador report

Ambassador report

[Ambassador report] Oyster mushroom cultivation

by Dibya Bhatta | 16-01-2020 13:37 Comments 8 Comments recommendations 0 recommendations

I will explain you how we can cultivate Oyster mushroom at home(Step by step guide and I have also prepared a video to demonstrate the process: How to cultivate Oyster mushroom)
You can subscribe to my channel for similar videos!✌️
Here is brief but detail process of oyster mushroom cultivation---
Cultivation practice of Pleurotus spp.
• Pleurotus spp. can be grown on paddy straw, wheat straw, 
banana leaves etc.
• Substrate for cultivation: paddy/wheat straw etc
• Spawn
• Pasteurization/sterilization unit
• Polythene bags 
• Supplements (gram flour/saw dust etc.) (optional)
• Cultivation room (with proper temperature, light, 

1. Selection of Substrate
• Selection of substrates for Pleurotus cultivation is most
important aspect.
• Pleurotus can be cultivated on various substrates which are
• It can be cultivated on pure substrate or combination of
different substrates.
• It can be grown on various agricultural waste materials.
• They grow well on a mixture of saw dust and other
combination of tropical wastes such as corn cobs and
leaves, cotton waste, sugarcane bagasse and leaves.
• Paddy straw was used as a substrate for the cultivation of
oyster which is a common practice in Nepal.

2. Sterilization
• Before sterilization rice straw should be chopped to about 5 cm or
smaller length.
• The chopped straw should be soaked in water overnight. For the best
result the straw should be immersed in boiling water drained off.
• If wheat straw is used, it should be softened by composting for a week.
• Sterilization can be done with the help of drum.
• There should be 4”×6” water in the drum where three or four bricks
should be kept upright position; perforated round plate of tin should
be kept on bricks.
• Then, the drum should be filled with moist straw.
• It should be steamed for half an hour to kill all insects and wild fungus.
• After steam sterilization it should be cooled either in drum itself or in
big plastic bag.
• In context of Nepal farmers are using this method for sterilization as they
don’t have any other well equipment for sterilization.
• So complete sterilization can not be assured by this method.
• Most of farmers in our country are not taking care during sterilization.
• So, they have to face contamination problem.
• For Pleurotus cultivation straw should be well sterilized.
3. Inoculation:
• Cultivation of Pleurotus is usually carried out in transparent
polythene bags.
• It is not necessary the size of plastic bag should be fixed.
• However experience show that small bag is good for summer
season and large size bag is good for winter season.
• Plastic bag filling method is popular in Nepal.
• For inoculation of the spawn; it would be good to have bag size -
14” × 22” (in summer) or 16” × 26”(in winter).
• Spawn should be spread in every layer of straw of 6-8 cm height
and pressed the straw slightly to make bag compact.
• After filling the bag with spawn and straw, mouth of the bag
should be tied with thread.
• Small holes should be made for aeration.
• Inoculation should be done with clean hand.
• Ones should be careful during inoculation to minimize the
possibility of contamination as far as possible.
• It would be better to have a separate room for inoculation.
4. Incubation:
• It is the process of providing suitable environmental
conditions to run mycelium well in substratum.
• During incubation, light is not required, however,
make sure the bags have plenty of fresh air.
• The packed bags or blocks are incubated in a well
ventilated room where uniform temperature of 25ºC is
• The area was all covered with wet jute sack to provide
it moist condition. Water was timely sprinkled.
• In Nepal, the most dangerous problem during
incubation period is Green Mold.
5. Observation
• Mushroom, then should be observed thoroughly,
following changes can be seen,
• 3rd day: no changes may be seen except some dew
collected at the top of bag.
• 6th day: minute threads of mycelium spread around
the spawned grain can be seen.
• 10th day: White mycelial thread can cover larger
• 15th day: mycelium may extent to larger areas but
nearly same as in 10th day.
• 25th day: now the fungus cover most of the part and
at that day the plastic covered can be removed and
placed in another room for further production.
6. Harvesting:
• During reproductive phase ventilation should be provided
so that fresh air can flow easily.
• Temperature and humidity should be maintained.
• Light water spray is necessary two times per day.
• When temperature is low, fruiting body are ready to harvest
at about 7-8 days but when temperature is high it may get
ready within 3-4 days.
• Harvesting is done when the cap has the diameter of 8-10
• Picking is done by twisting gently so that it is pulled out
without leaving any stalk and also the nearby fruiting bodies
are not disturbed.
• When the base of the stipe is deeply immersed within the
straw, cutting the base of stipe with Sharpe knife can be
In this way the mushroom gets ready to come to our plates.
T hus cultivating mushrooms on your own will help you and your family to feed the healthy and organic vegetable.you can even cutivate this mushroom in single use plastic bottle so the waste can also be minimized.You can also cultivate mushroom on commercial scale and feed your neighbor with healthy veggies.
Happy cultivation!
Have a great day!


Dibya Bhatta

  • Dibya Bhatta says :
    Thank you so much for your beautiful comments!! :)
    Posted 23-01-2020 17:48

Kushal Naharki

  • Kushal Naharki says :
    Hello dibya didi :)

    I do hope that you are fine and doing great with your works.
    Thank you for your report about oyster mushroom cultivation

    Green Cheers from Nepal :)
    Keep writing great reports.
    We are eager to read more reports from you.

    Kushal Naharki

    Posted 20-01-2020 04:58

Lisa Mentor

  • Lisa Mentor says :
    Hey Dibya!
    this is your mentor Lisa.

    Some great tips on cultivating oyster mushroom you have here!
    I personally have never cultivated any vegetables in my house so I think your tips would come in very handy in the day I first plant my vegies!
    Oyster mushrooms are very popular in Korea as well and I believe that Korea is not the single Asian country that eat it. I think your article could be a real help to those nations:)

    Thanks for sharing your tips!
    Posted 18-01-2020 21:50

Pooja Gyawali

  • Pooja Gyawali says :
    Hello Divya dd
    So informative.. Well written dd
    Green Cheers!!!
    Posted 17-01-2020 01:42

Prabha Bhusal

  • Prabha Bhusal says :
    Hello Divya dd,
    This steps can be carried out at our own home for the cultivation of Oyster. Mushroom.We can get great economic return from this cultivation.Thank you for giving all the information about the process of Cultivation.
    Green cheers!
    Posted 16-01-2020 20:41

Hyeongmin Mentor

  • Hyeongmin Mentor says :
    Hello Dibya

    I really like oyster mushrooms, but didn't know that I can cultivate them at home. By reading your report, I learned that oyster mushrooms can be cultivated with not much complicated setting, so I'm planning to try it one day.

    Thank you for the informative report!
    Posted 16-01-2020 18:14

Shreya Aryal

  • Shreya Aryal says :
    Hello Dibya Dd,
    I still remember of our last years mushroom cultivation.You have explained every steps in detail and so beautifully.
    Green Cheers,
    Posted 16-01-2020 16:48

Paras Kunwar

  • Paras Kunwar says :
    great technique

    Posted 16-01-2020 15:27

Post a comment

Please sign in